HPLC working Things To Know Before You Buy
a values, the pH with the mobile phase has another effect on Each individual solute’s retention time, letting us to find the the best possible pH for effecting a whole separation in the 4 solutes.. HPLC separation of a mixture of flavonoids with UV/Vis detection at 360 nm and, inside the inset, at 260 nm. The choice of wavelength influences Each